Determination of mtDNA Sequences by Using Novel Primers to Establish Intraspecific and Phylogenetic Relations of Hungarian Isophya (Orthoptera, Tettigoniidae) Species
E. Vadkerti *
Faculty of Water Science, National University of Public Service, H-6500 Baja, Bajcsy-Zsilinszky Str. 12-14, Hungary
P. Putnoky
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
G. Hoffmann
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
F. Antal
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
M. Boros
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
K. Götzer
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
P. Kisfali
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
I. Mészáros
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
K. Nyárády
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
S. Stranczinger
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
A. Szloboda
Faculty of Sciences, University of Pécs, H-7601 Pecs, Ifjúsag Str. 6, Hungary
S. Farkas
Faculty of Agricultural and Environmental Sciences, Kaposvár University, H-7400 Kaposvár, Guba Sándor Str. 40, Hungary
T. Karches
Faculty of Water Science, National University of Public Service, H-6500 Baja, Bajcsy-Zsilinszky Str. 12-14, Hungary
I. Matrai
Faculty of Water Science, National University of Public Service, H-6500 Baja, Bajcsy-Zsilinszky Str. 12-14, Hungary
Z. Varga
Department of Evolutionary Zoology and Human Biology, Faculty of Sciences, University of Debrecen, H-4010 Debrecen, Egyetem Sq. 1, Hungary
K. Pecsenye
Department of Evolutionary Zoology and Human Biology, Faculty of Sciences, University of Debrecen, H-4010 Debrecen, Egyetem Sq. 1, Hungary
*Author to whom correspondence should be addressed.
Abstract
Novel gene specific primers were designed for coding sequences of a part of 16S ribosomal RNA encoding gene and a piece of ND1 gene, separated by a short stretch of DNA that encodes a tRNA-Leu gene for Isophya species.
Keywords: Isophya, mtDNA, ND1 gene, PCR primers, 16S RNA gene