Kinetic and Morpho-Functional Analyses of Refrigerated and Cryopreserved Semen of 5/8 Girolando Bulls
Maiana Silva Chaves *
Department of Veterinary Medicine, Laboratory of Reproductive Biotechniques, Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil and Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
Satish Kumar
Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
José Carlos Ferreira de Silva
Department of Veterinary Medicine, Laboratory of Reproductive Biotechniques, Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil.
Marcos Antônio Lemos de Oliveira
Department of Veterinary Medicine, Laboratory of Reproductive Biotechniques, Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil.
Irving Mitchell Laines Arcce
Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
Natanael Aguiar Braga Negreiros
Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
Ana Flávia Bezerra da Silva
Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
Luciana Magalhães Melo
Centre for Education, Science and Technology of the Inhamuns Region, State University of Ceará (UECE), Tauá, CE, Brazil.
Vicente José de Figueirêdo Freitas
Laborotory of Physiology and Reproduction Control, Faculty of Veterinary Medicine, State University of Ceara (UECE), Fortaleza, CE, Brazil.
Claudio Coutinho Bartolomeu
Department of Veterinary Medicine, Laboratory of Reproductive Biotechniques, Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to investigate the effects of refrigeration and cryopreservation on the sperm kinetic and morpho-functional parameters of 5/8 Girolando bulls. Eleven ejaculates from each bull were collected, diluted, and divided into two portions: one portion was subjected to refrigeration at 5ºC for 24 and 48 hours, while the second portion underwent cryopreservation(-196ºC). Post-thawing, various kinetic parameters were analyzed using the Computer Assisted Sperm Analysis (CASA) system. These parameters included total motility (TM), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness coefficient (STR), wobble coefficient (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF). Additionally, plasma membrane integrity (PMI), acrosomal membrane integrity (AMI), mitochondrial membrane potential (MMP), and chromatin condensation (CC) were examined for morpho-functional analyses. The results indicated that kinetic parameters (TM, PM, VCL, VSL, VAP) and morpho-functional analyses (PMI, MMP) differed significantly (P < 0.05) between cryopreserved and refrigerated semen, as well as other parameters such as LIN, ALH, and BCF across all groups. The acrosomal membrane integrity showed no differences, but CC varied significantly among all groups. In conclusion, semen from 5/8 Girolando bulls can be preserved for up to 48 hours at 5ºC, providing an alternative for the short-term use of genetic material from this breed.
Keywords: Bovine, CASA, crossbred, frozen spermatozoa, zebu semen