Evaluation of the Effect of Oxidative Stress Generated by Cadmium (Cd) in the Yeast Saccharomyces cerevisiae
Fadila Khaldi *
Laboratory of Microbiology, Department of Biology, Faculty of Natural Science and Life, University of Mohamed Cherif Messaadia, PBOX1553, Souk-Ahras, 41000, Algeria
Nedjoud Grara
Department of Biology, Faculty of Natural Science and Life and Sciences of the Earth and the Universe, University of 8 May 1945, PBOX 401, Guelma, 24000, Algeria
Amina Boulahlib
Laboratory of Microbiology, Department of Biology, Faculty of Natural Science and Life, University of Mohamed Cherif Messaadia, PBOX1553, Souk-Ahras, 41000, Algeria
Imen Tebib
Laboratory of Microbiology, Department of Biology, Faculty of Natural Science and Life, University of Mohamed Cherif Messaadia, PBOX1553, Souk-Ahras, 41000, Algeria
Khouloud Boukehili
Laboratory of Microbiology, Department of Biology, Faculty of Natural Science and Life, University of Mohamed Cherif Messaadia, PBOX1553, Souk-Ahras, 41000, Algeria
*Author to whom correspondence should be addressed.
Abstract
Inhibition of the mitochondrial electron transfer chain and induction of reactive oxygen species (ROS) production are one of the roots of cadmium (Cd) toxicity. Our work concerns the study of the effects of a heavy metal; Cadmium (Cd) on the biochemical parameters, of microorganism bioaccumulator and bioindicators of pollution Saccharomyces cerevisiae. The main results show that the presence of Cadmium affects the growth of yeast, and biochemical assays reveal an increase significant of total protein and a decrease in the rate of carbohydrates. With respect to biomarkers, we note a significant decrease in the level of GSH. The results of this study demonstrate a metabolic imbalance associated oxidative stress in Saccharomyces cerevisiae.
Keywords: Saccharomyces cerevisiae, heavy metals, cadmium, biomarkers, pollution, GSH