Efficacy of Various Concentrations of Honey Solution in the Fixation of Some Selected Tissues in Wistar Rats
U. Abubakar *
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
A. Ishaq
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
O. O. Okechi
Department of Medical Laboratory Science, Abia State University, Uturu, Nigeria.
A. Umar
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
R. I. Tsamiya
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
M. O. Mohammed
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
A. T. Muhammad
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
. Mohammed
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
U. Mohammed
Department of Histopathology, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
N. Okorie
Department of Medical Laboratory Science, Faculty of Health Sciences, Ebonyi State University, Nigeria.
F. A. Dogondaji
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
S. M. Sani
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
A. Abdulazeez
Department of Histopathology, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
S. Y. Ma’aruf
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
H. Kabir
Department of Histopathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
B. A. Bello
Department of Chemical Pathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
N. A. Idris
Department of Medical Laboratory Science, General Hospital Maiyama, Maiyama Local Government, Kebbi State, Nigeria.
K. H. Magaji
Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
A. H. Mohammed
Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
M. U. Kalgo
Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
A. I. Bagudo
Department of Haematology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Honey is thought to preserve the cells by preventing autolysis and putrefaction. Thus, studies have shown that honey has a good preventive putrefactive property mainly because honey contains seven tetracycline derivatives, fatty acids, lipids, amylases and ascorbic acid and hydrogen peroxide. Therefore, honey is used as an agent for preventing autolysis and putrefaction.
Aim: This research aims to investigate the efficacy of some concentrations of the honey solution in fixation of some tissues (Such as kidney, liver, heart, lungs and muscle).
Methodology: A Wistar rat was sacrificed by vertical abdominal incision and the above-mentioned organs ware fixed in 10% formalin (positive control), 10% honey and 20% honey this grouped the research specimen into 3 groups. Histological examinations of these organs were carried out.
Results: Almost all the histological sections fixed in honey solution revealed normal nuclear as well as a cytoplasmic outline as compared with formalin-fixed organs, except for muscle which shown some level of swollen and fragmenting tissue.
Keywords: Honey solution, Fixation various concentrations and some selected tissues.